Effects of Photofrin II on adults of Eretmapodites quinquevittatus.

This research was conducted to further assess the efficacy of photosensitizers as adult mosquito control agents. Fourth-stage larvae of Eretmapodites quinquevittatus were fed Photofrin porfimer sodium (PII) (< or = 3.75 microg/ml) and the resulting adults (parentals) were photoirradiated.

Site of Photofrin II photosensitization in larvae of Eretmapodites quinquevittatus Theobald.

Photofrin II (PII) accumulates and fluoresces in the alimentary canal, anal papillae, malpighian tubules, rectum, and gastric caeca at greater concentrations (>10 microg/ml) and accumulates (fluoresces) in (along) the alimentary canal only at lower concentrations (< or =10 microg/ml). Initial experiments with larvae exposed to different osmotic conditions and PII indicated that cells of osmoregulation were most susceptible to life-threatening cell damage. Larvae that lacked anal papillae and were exposed to different osmotic conditions and PII indicated that cells of osmoregulation may not be the most deleterious site for cell damage.

Photosensitization of Eretmapodites quinquevittatus Theobald (Diptera: Culicidae) eggs and larvae with Photofrin II.

The susceptibility of Eretmapodites quinquevittatus eggs and larvae to photosensitization with Photofrin II (PII) was determined. Hatching rates of eggs (5 min to 2 h after oviposition with no PII exposure) exposed to solutions of PII (90 micrograms/ml) in the dark for 12 h, before photoirradiation for 72 h. were very similar to those of untreated controls (P > 0.

Fecundity, fertility, and developmental stage survivorship of Eretmapodites quinquevittatus.

This laboratory study analyzed fecundity, fertility (percentage hatched), and developmental stage survivorship of Eretmapodites quinquevittatus. Fecundity for females averaged 190.4 eggs, whereas paired (one male/female pair) females averaged 100.