[Progesterone - regulator of T-lymphocytes cytotoxicity].

Progesterone plays important the role in the regulation of the immune system during pregnancy. We examined the effect of progesterone on the cytotoxic activity of T-lymphocytes in a model system Jurkat cells. Jurkat cells were stimulated with 50 μg/ml of phytohemaglutinin A (PHA) at 370C for 5 minutes.

[The role of neuroendocrine mediators in regulatory activity of T-cells].

The aim of the study was to establish the role of some neuroendocrine mediators (agonists and antagonists of β-adrenergic receptors, progesterone) in regulating T-cells activity. Studies conducted on the culture of leukemiatransformed T-cells (Jurkat cells) (DSMZ-Deutshe Sammulung von Mikroorganismen und Zellkulturen (Germany)). Jurkat Cells (4 x 105 cells/ml) stimulated with 50 µg/ml fitogemaglutinin A (PHA) at 370С for 5 minutes and then incubated for 24 hours alone, or together with β- adrenergic receptors agonist izopretonolom (at dose of 10-5 M, 10-6 M), an antagonist, propranolol (dose of 10-5 M, 10-6 M) and progesterone (dose 0,07 µl, 0,7 µl) added to the incubation medium.