Depletion of TP53 in Human Pluripotent Stem Cells Triggers Malignant-Like Behavior.

Human pluripotent stem cells (hPSCs) tend to acquire genetic aberrations upon culture in vitro. Common aberrations are mutations in the tumor suppressor TP53, suspected to confer a growth-advantage to the mutant cells. However, their full impact in the development of malignant features and safety of hPSCs for downstream applications is yet to be elucidated.

A Postanesthesia Care Unit Parathyroid Hormone-Based Protocol for Managing Postthyroidectomy Hypocalcemia.

Introduction: Hypocalcemia occurs in 20%-40% of total thyroidectomy cases, traditionally requires 1-2 ds of hospitalization for management. This study examines the extent of hypocalcemia following a postanesthesia care unit (PACU) parathyroid hormone (PTH)-based protocol after outpatient thyroidectomy.

Methods: Patients who underwent total or completion thyroidectomy for non-Graves' disease at a single institution between December 2015 and September 2023 were included.

Empowering Futures: The Impact of Surgical Research Pipeline Programs on Undergraduate Career Development.

Purpose: Surgical research pipeline programs provide a unique insight into academic surgery, offering a distinctive opportunity for aspiring future surgeons and medical professionals to identify suitable mentorship. The objective of this study was to determine the impact of an academic summer research, shadowing, and mentorship program on undergraduate students with interest in the fields of medicine and surgery.

Methods: We conducted a convergent mixed methods study on undergraduate students who participated in the Surgery Undergraduate Research Experience (SURE) program from 2017 to 2022, excluding the 2020 cohort as their experience was highly impacted by COVID-19.

CRL3 ubiquitin ligase and Integrator phosphatase form parallel mechanisms to control early stages of RNA Pol II transcription.

Control of RNA polymerase II (RNA Pol II) through ubiquitylation is essential for the DNA-damage response. Here, we reveal a distinct ubiquitylation pathway in human cells, mediated by CRL3, that targets excessive and defective RNA Pol II molecules at the initial stages of the transcription cycle. Upon ARMC5 loss, RNA Pol II accumulates in the free pool and in the promoter-proximal zone but is not permitted into elongation.

NET guidelines for white patients may not fit Asian patients.

Introduction: Pancreatic neuroendocrine tumors (pNETs) are slow growing, malignant tumors that show different survival outcomes by race. Current size-based guidelines were largely developed in White patients. Our aim was to investigate tumor size and incidence of lymph node metastasis (LNM) between White and Asian pNET patients to evaluate generalizability of established guidelines.