Publications by authors named "A M Diks"
Neither immunization nor recovery from natural infection provides life-long protection against Bordetella pertussis. Replacement of a whole-cell pertussis (wP) vaccine with an acellular pertussis (aP) vaccine, mutations in B. pertussis strains, and better diagnostic techniques, contribute to resurgence of number of cases especially in young infants.
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- They created a new method called AGI to help scientists quickly and easily identify different types of blood cells, especially B-lymphocytes and plasma cells, without needing a lot of training.
- To test this method, they used data from blood samples of healthy people and patients, analyzing them with special antibodies.
- The new AGI method showed results that were very similar to the old way of analyzing cells but took much less time to get those results.
Background: The rs72824905 single-nucleotide polymorphism in the PLCG2 gene, encoding the p.P522R residue change in Phospholipase C gamma 2 (PLCγ2), associates with protection against several dementia subtypes and with increased likelihood of longevity. Cell lines and animal models indicated that p.
View Article and Find Full Text PDFBACKGROUNDTo date, only limited data are available on the mechanisms of protection against colonization with Bordetella pertussis in humans.METHODSIn this study, the cellular responses to B. pertussis challenge were monitored longitudinally using high-dimensional EuroFlow-based flow cytometry, allowing quantitative detection of more than 250 different immune cell subsets in the blood of 15 healthy donors.
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- * A study evaluated 62 antibodies to improve the detection of IMC populations, ultimately selecting 16 for two effective antibody combinations aimed at clinical and research applications.
- * The final 11- and 14-color antibody combinations allowed for precise detection of 19 and 23 IMC populations, respectively, with consistent results across different types of instruments and conditions, though delays in sample processing impacted absolute cell counts.