A single-particle analysis method for detecting membrane remodelling and curvature sensing.

In biology, shape and function are related. Therefore, it is important to understand how membrane shape is generated, stabilised and sensed by proteins and how this relates to organelle function. Here, we present an assay that can detect curvature preference and membrane remodelling with free-floating liposomes using protein concentrations in physiologically relevant ranges.

Acclimation to medium-level non-lethal iron limitation: Adjustment of electron flow around the PSII and metalloprotein expression in Trichodesmium erythraeum IMS101.

The aim of this study was to investigate how acclimation to medium-level, long-term, non-lethal iron limitation changes the electron flux around the Photosystem II of the oceanic diazotroph Trichodesmium erythraeum IMS101. Fe availability of about 5× and 100× lower than a replete level, i.e.

Mechanism of Hydrogen Peroxide Formation on Sprayed Water Microdroplets.

How is HO formed in sprayed water is not well understood. It is believed to involve the association of HO radicals spontaneously generated from HO ions by internal electric fields on the surface of neutral microdroplets. Spraying water actually creates charged microdroplets carrying either excess OH or H intrinsic ions that repel each other toward the very surface.

Comment on "Liquid-Gas Interface of Iron Aqueous Solutions and Fenton Reagents".

A recent Letter in this Journal (Gladich et al. 13, 2994-3001) reported that photoelectron spectroscopy could not detect any products on the surface of aqueous Fe jets dosed with gaseous hydrogen peroxide. The Letter however concluded that Fe(aq) ions react with HO(g) at the water-vapor interface to produce the same Fe + HO products as their reaction with HO(aq) in bulk water.

Role of Ferryl Ion Intermediates in Fast Fenton Chemistry on Aqueous Microdroplets.

In the aqueous environment, Fe ions enhance the oxidative potential of ozone and hydrogen peroxide by generating the reactive oxoiron species (ferryl ion, FeO) and hydroxyl radical (·OH) via Fenton chemistry. Herein, we investigate factors that control the pathways of these reactive intermediates in the oxidation of dimethyl sulfoxide (MeSO) in Fe solutions reacting with O in both bulk-phase water and on the surfaces of aqueous microdroplets. Electrospray ionization mass spectrometry is used to quantify the formation of dimethyl sulfone (MeSO, from FeO + MeSO) and methanesulfonate (MeSO, from ·OH + MeSO) over a wide range of Fe and O concentrations and pH.