[Rapid technic for preparation of thyroid ribonucleic acids without phenol].

Thyroid tissue was homogenized in 2 M LiCl. The homogenate was alloued to stand 1 h 30 min at 2 degrees C and then centrifuged. The pellet was suspended in 5% triisopropylnaphthalene sulfonic acid (the sodium salt), 0.

[Isolation and characterization of beef thyroid giant RNA].

Total HMW-RNAs were prepared by three different methods (method with phenol, method with NaClO4, method without phenol using Ultrogel AcA 22 filtration). Giant RNAs were obtained in the void volume by filtration on Sepharose 2B. The giant RNAs/total HMW-RNA ratio is higher (6.

Ribosomal and transfer RNA contents of the normal and pathologic human thyroid gland.

One hundred and seventy seven pieces of normal or pathologic thyroid tissue from 17 patients were assayed for rRNA, tRNA and DNA content. The tRNA/DNA and rRNA/DNA ratios in pathologic tissue were statistically compared with the same ratios in normal tissue. In toxic adenoma (5 cases) and anoplastic cancer (2 cases), both ratios were increased.