Deep eutectic solvents with solid supports used in microextraction processes applied for endocrine-disrupting chemicals.

The determination of endocrine-disrupting chemicals (EDCs) has become one of the biggest challenges in Analytical Chemistry. Due to the low concentration of these compounds in different kinds of samples, it becomes necessary to employ efficient sample preparation methods and sensitive measurement techniques to achieve low limits of detection. This issue becomes even more struggling when the principles of the Green Analytical Chemistry are added to the equation, since finding an efficient sample preparation method with low damaging properties for health and environment may become laborious.

Exposure scenario and risk assessment of infants and newborns to bisphenols and their derivatives from diapers.

Newborns and infants are more sensitive to harmful compounds such as bisphenols and their derivatives because of their not fully developed detoxification mechanism. Exposure to these substances can lead to developmental problems and health consequences in adulthood. Since disposable baby diapers are used from the first days of life and remain in contact with the baby skin, it seems important to monitor the levels of endocrine disrupting chemicals (EDCs) in such products.

Decreased Activity of Blood Acid Sphingomyelinase in the Course of Multiple Myeloma.

Acid sphingomyelinase (aSMase) is involved in the generation of metabolites that function as part of the sphingolipid signaling pathway. It catalyzes the breakdown of sphingomyelin into ceramide, a bioactive lipid that, among other roles, is involved in regulation of apoptosis. Dry drop blood test (DBS) and colorimetric 2-step enzymatic assay were used to assess the activity of human blood aSMase, beta-galactosidase, and beta-glucosidase, these enzymes are lysosomal hydrolases that catalyze the degradation of related sphingolipids, of sphingolipid signaling molecules.

Fatty acids and sphingolipids profile in the blood plasma of experienced divers in response to hyperbaric exposure.

Introduction: Hyperbaric exposure mimics air-breathing scuba diving, which is reaching enormous popularity around the world. The diver's body is subjected to a broad range of divergent effects exerted by, e.g.

Unexpected profile of sphingolipid contents in blood and bone marrow plasma collected from patients diagnosed with acute myeloid leukemia.

Background: Impaired apoptotic pathways in leukemic cells enable them to grow in an uncontrolled way. Moreover, aberrations in the apoptotic pathways are the main factor of leukemic cells drug resistance.

Methods: To assess the presence of potential abnormalities that might promote dysfunction of leukemic cells growth, HPLC system was used to determine sphingosine (SFO), sphinganine (SFA), sphingosine-1-phosphate (S1P) and ceramide (CER) concentration in the blood collected from patients diagnose with acute myeloblastic leukemia (AML; n = 49) and compare to values of control (healthily) group (n = 51).