Asymmetric D-A-D' Ratiometric Molecule for Highly Specific Hypochlorous Acid Detection.

Hypochlorous acid exists as HClO in acidic conditions and as ClO in alkaline conditions, posing a significant challenge for differentiation due to their strong and closely similar oxidative reaction activities. Addressing this challenge, our study presents an asymmetric donor-acceptor-donor' (D-A-D') molecular architecture for the design of a fluorescent probe ( NPs) that demonstrates exceptionally high specificity toward HClO alongside an optimized ratiometric response. The incorporation of the strong electron acceptor 2-(diphenylmethylene)malononitrile (A) modulates the reducing ability of the phenothiazine recognition site, adjusting the probe's oxidation potential to an intermediate level between HClO and ClO.

Seq2Topt: a sequence-based deep learning predictor of enzyme optimal temperature.

An accurate deep learning predictor is needed for enzyme optimal temperature (${T}_{opt}$), which quantitatively describes how temperature affects the enzyme catalytic activity. In comparison with existing models, a new model developed in this study, Seq2Topt, reached a superior accuracy on ${T}_{opt}$ prediction just using protein sequences (RMSE = 12.26°C and R2 = 0.

Evaluation of Vitamin D Supplementation in Critically Ill Patients-A Narrative Review of Randomized Controlled Trials Published in the Last 5 Years.

The prevalence of vitamin D deficiency among intensive care unit (ICU) patients is potentially associated with an increased risk of mechanical ventilation, sepsis, prolonged hospital stays, and mortality. Although ICU patient care has significantly improved in recent years, the role of vitamin D supplementation remains under investigation. A literature review was conducted using PubMed, Web of Science, Embase, and Cochrane databases, focusing on randomized controlled trials published in the past five years on vitamin D supplementation in adult ICU patients.

Dys-regulated phosphatidylserine externalization as a cell intrinsic immune escape mechanism in cancer.

The negatively charged aminophospholipid, phosphatidylserine (PS), is typically restricted to the inner leaflet of the plasma membrane under normal, healthy physiological conditions. PS is irreversibly externalized during apoptosis, where it serves as a signal for elimination by efferocytosis. PS is also reversibly and transiently externalized during cell activation such as platelet and immune cell activation.