Structure and catalytic activity of the SAM-utilizing ribozyme SAMURI.

Ribozymes that catalyze site-specific RNA modification have recently gained increasing interest for their ability to mimic methyltransferase enzymes and for their application to install molecular tags. Recently, we reported SAMURI as a site-specific alkyltransferase ribozyme using S-adenosylmethionine (SAM) or a stabilized analog to transfer a methyl or propargyl group to N of an adenosine. Here, we report the crystal structures of SAMURI in the postcatalytic state.

Over 400 food resources from Brazil: evidence-based records of wild edible mushrooms.

Many species of mushroom-forming fungi have been harvested in the wild and used for food and medicine for thousands of years. In Brazil, the knowledge of the diversity of wild edible mushrooms remains scattered and poorly studied. Based on new samples, bibliographic records revision, and searches through the GenBank, we recorded 409 species of wild edible mushrooms in Brazil, of which 350 can be safely consumed and 59 are edible but with conditions.

Cadaveric validation of markerless tracking using weightbearing computed tomography versus conventional computed tomography imaging techniques.

This study aimed to validate the use of weightbearing computed tomography against conventional computed tomography and against bead tracking for markerless tracking of key foot and ankle bones. A left cadaveric limb was implanted with tantalum beads and underwent conventional computed tomography and weightbearing computed tomography scanning, followed by biplane fluoroscopy motion capture to simulate gait. Bone models from conventional computed tomography and weightbearing computed tomography were compared for surface differences and kinematic analysis across six joints.

Single-pixel microscopy with optical sectioning.

Imaging with single-pixel detectors offers a valuable alternative to the conventional focal plane array strategy, especially for wavelengths where silicon-based sensor arrays exhibit lower efficiency. However, the absence of optical sectioning remains a challenge in single-pixel microscopy. In this paper, we introduce a single-pixel microscope with optical sectioning capabilities by integrating single-pixel imaging (SPI) techniques with structured illumination microscopy (SIM) methods.

Making a Splash: A Closer Look at Early Specialization in Competitive Diving.

This review article explores the multifaceted aspects of early specialization in diving, focusing on its implications for injury prevalence, performance, and mental health. It highlights the increasing trend of early specialization driven by ambitions for collegiate and Olympic success, despite recommendations advocating for diversification. Divers are at risk for acute and overuse injuries, particularly during high-velocity water entries; however, data on injury patterns, especially among elite divers and early specializers, remains scarce.