[In vitro induced activity of polymorphonuclear leukocyte myeloperoxidase at different stages of opisthorchis invasion and at stages of a different rehabilitative period].

Polymorphonuclear leukocytes from 115 patients with opisthrochiasis at its different (acute and chronic) stages were studied before and 1-3 days, 1-3 months, and 1-2 years after antihelminthic therapy with bilthricide. The functional activity of the cells in question was evaluated following an hour load with different drugs: bilthricide, ascorbic acid, decaris, cyclophosphane, doxycycline in a final concentration of 2.5 x 10(-5) mole/l.

[Impact of anthelminthic therapy for opisthorchiasis on the biochemical parameters of hepatic function].

A response of a complex of biochemical parameters of hepatic function to therapeutic exposures was studied at different stages of development of Opisthorchis invasion. The activity of AST, ALT, GGT, GGT, AP, AM, and CE and the concentration of bilirubin, cholesterol, and glucose in the sera from 232 patients with acute or chronic opisthorchiasis were studied. Their study was conducted in several steps: before treatment, after a course of pathogenetic therapy, 1-3 days after antihelminthic therapy with bilthricide and azinox, in early (following 1-3 months) and late (following more than 6 months) residual periods.

[Increase of lysosomal cationic proteins of polymorphonuclear leukocytes as a response to opisthorchis invasion].

Cytochemistry was used to study the level of lysosomal cationic proteins of polymorphonuclear peripheral leukocytes in 25 and 20 patients with acute and chronic opisthorchiasis, respectively, before treatment. Comparison groups included patients with chronic viral hepatitis C, bronchial asthma, as well as apparently healthy individuals free of Opisthorchis invasion. That the ratios of an increase in the intracellular content of lysosomal cationic proteins to that in the control individuals were found to be 13.

[The populational structure of peripheral lymphocytes in patients in the acute phase of opisthorchiasis].

The lymphocytic populations of the immune system were studied in patients diagnosed as having acute opisthorchiasis before and 1-3 days after treatment with biltricide in a dose of 60 mg/kg body weight. The acute phase of opisthorchiasis is characterized by the activation of B lymphocytes and immunological cellular reserve, by depressed T-lymphocytic population with a decreased helper inductor and suppressive killer potential. Three days after dehelminthization, there was a trend for immunoregulatory T cells to become normal.

[Biochemical characteristics of hepatic functions in different clinical forms of chronic opisthorchiasis].

The main biochemical indices of hepatic functions (the activities of alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, alkaline phosphatase, alpha-amylase, choline esterase and the concentrations of total bilirubin, cholesterol, and glucose) were studied in the sera of 256 patients with chronic opisthorchiasis. It was found that with diseases manifested in different clinical forms (cholangitis, cholecystitis, cholangiocholecystitis, cholangiohepatitis, cholecystitis in combination with pancreatitis), most study indices are within the normal ranges, but significantly differ from the means in a group of apparently healthy individuals. The findings suggest that such clinical forms of opisthorchiais as cholangiocholecystitis and cholangiohepatitis are characterized by manifestations of cytolysis and cholestasis, as cholecystitis is manifested by cytolysis, as cholecystitis in combination with pancreatitis, by cholestasis, and as cholangitis, by cholestasis and hepatic cell insufficiency.