Mol Gen Mikrobiol Virusol
December 1988
Biological as well as physicochemical properties of Bacillus thuringiensis bacteriophages "17" and "7/13" having C2-morphology and isolated from factory phagolysates were studied. The bacteriophages are identical in the lytic spectrum++, morphology, size, GC-content, have the same buoyant density. The physical map for restriction endonucleases EcoRI, HindIII, SalGI and MvaI has been constructed of the bacteriophages DNA.
View Article and Find Full Text PDFElectrophoretic mobility of Escherichia coli cells exposed to various doses of UV-radiation was investigated. The method of free flow electrophoresis was used to study a correlation between membrane protein charge and cell surface electric charge. The change in the cell surface charge and electrophoretic motility was associated with the damage to membrane proteins and the survival of UV-irradiated bacteria.
View Article and Find Full Text PDFA change in the electro-orientation spectrum followed by a change in the electric characteristics of bacterial cells had been measured when the barrier function of the cytoplasmic membrane was disordered under the action of damaging physical and chemical factors. Analysis of changes in the spectra at a frequency of 10(4) to 10(7) Hz allows one to estimate the degree of cell damage in quantitative terms. The paper presents the results of comparing three procedures for such an estimation: inoculation onto solid growth media; linear regression analysis of an electro-orientation spectrum; comparison of the electro-orientation effect at a high frequency (ca.
View Article and Find Full Text PDFThe effect of the mutagene nitrosoguanidine (N-methyl-N'-nitro-N-nitrosoguanidine) on the growth of Bacillus thuringiensis subsp. galleriae st. 69/6 was being studied.
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