A concept of natural genome reconstruction.Part 2. Effect of extracellular double-stranded DNA fragments on hematopoietic stem cells.

In this part of the study, the first component of the concept of "natural genome reconstruction" is being proven. It was shown with mouse and human model organisms that CD34+ hematopoietic bone marrow progenitors take up fragments of extracellular double-stranded DNA through a natural mechanism. It is known that the process of internalization of extracellular DNA fragments involves glycocalyx structures, which include glycoproteins/protein glycans, glycosylphosphatidylinositol-anchored proteins and scavenger receptors.

Stimulation of mouse hematopoietic stem cells by angiogenin and DNA preparations.

Immature hematopoietic progenitors are a constant source for renewal of hemocyte populations and the basic component of the tissue and cell repair apparatus. A unique property of these cells of internalizing extracellular double-stranded DNA has been previously shown. The leukostimulatory effect demonstrated in our pioneering studies was considered to be due to the feature of this cell.

Lithium-Modified Sorbent: Effects on Hemostatic Responses In Vitro.

The effects of a lithium-modified sorbent based on aluminum oxide and polydimethylsiloxane (AlO@PDMS/Li) and a lithium-free sorbent (AlO@PDMS) on some indicators characterizing blood clotting under hemosorption conditions were compared in vitro. Sorbent AlO@PDMS/Li had significantly lower reactogenic effect on the blood passed through the column than the sorbent without lithium. This was seen from the degree of platelet reduction (66×10 vs 19×10/liter) as well as a less pronounced hypercoagulation shift in chronometric indicators.

Screening of the Promising Direct Thrombin Inhibitors from Haematophagous Organisms. Part I: Recombinant Analogues and Their Antithrombotic Activity In Vitro.

The success in treatment of venous thromboembolism and acute coronary syndromes using direct thrombin inhibitors has stimulated research aimed at finding a new anticoagulant from haematophagous organisms. This study deals with the comparison between hirudin-1 from (desirudin), being the first-known and most well-studied natural anticoagulant, along with recombinant analogs of haemadin from the leech , variegin from the tick , and anophelin from . These polypeptides were chosen due to their high specificity and affinity for thrombin, as well as their distinctive inhibitory mechanisms.

Minimally invasive mitral valve repair and ablation of concomitant atrial fibrillation in a patient with severe hemophilia A.

Minimally invasive mitral valve repair and ablation of atrial fibrillation, combined with FVIII level-controlled replacement therapy, can be safely performed in patients with severe hemophilia.