[A standard panel of human immunodeficiency virus-positive and -negative blood sera for assessing the sensitivity and specificity of diagnostic immunoenzyme test systems].

The materials on the development of a reference panel of HIV-positive and negative blood sera required for quality control of diagnostic enzyme-immunoassay systems are summarized. Ninety sera (50 HIV-antibody positive and 40 negative) were examined in 2 test systems of national and 3 test systems of foreign companies as well as by immune blotting and immunofluorescence tests. Each serum was characterized by optic density values and antibody titer (for positive sera).

[Immunobiological aspects of AIDS].

The growing pandemia of AIDS, which resulted in about 40,000 AIDS patients and about 3 million infected persons by the end of 1986, demands for the urgent creation of methods for diagnosis, prevention and treatment of the disease. The present paper analyzes the main aspects of AIDS immunobiology, i. e.

[Rapid immunoenzyme diagnosis of influenza in patients with various allergoses and bronchial asthma].

In an investigation carried out in the allergological clinic of the Tbilisi State Medical Institute in the period between two outbreaks of influenza, the presence of influenza antigen was determined in nasal washings taken from 127 patients with different allergoses and bronchial asthma by means of the enzyme immuno-assay with the use of the type-specific virion antigen of M1-protein. This method was found to be highly sensitive and to have some advantages over traditional methods used for the diagnosis of influenza. In patients with preasthma and different forms of bronchial asthma elevated susceptibility to influenza infection and its unfavorable influence on the clinical course of these pathological conditions were established.

[Islet cell surface autoantibodies: their immunoenzyme analysis using rat target cells].

A hard phase immunoenzymatic system for primary screening and subsequent quantitative determination of autoimmunobodies to the surface of islet cells (ASIC) was worked out using rat target cells. ASIC organ-specificity was confirmed by the absence of crossreactions with rat hepatocytes and splenocytes. The determination of the upper normal bound with 99% confidence interval combined with a high sensitivity of the method allowed an objective and significant assertainment of ASIC-positive sera even with a low level of antibodies.