COX-2 inhibitors prolong trauma-induced elevations of iris hyaluronan.

Purpose: To investigate whether and how treatment with COX-2 inhibitors influences hyaluronan responses to a standardized trauma, argon laser induced iritis, in rabbits.

Methods: Two different COX-2 inhibitors were used, SC-236 and rofecoxib. The drugs were administered orally, 6 mg/kg/day and 1.

Effects of sympathetic denervation on the hyaluronan content of the anterior segment in the normal and traumatized rabbit eye.

Purpose: To determine whether there is any involvement of sympathetic nerves in the regulation of ocular hyaluronan production in the normal and traumatized rabbit iris.

Methods: Unilateral sympathetic denervation was performed by removing the right superior cervical ganglion. Hyaluronan concentrations in the iris and aqueous were measured with a radiometric assay at various time intervals after denervation.

Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor.

Purpose: To monitor changes of endogenous hyaluronan in the iris tissue and aqueous humor after an isolated trauma to the iris by argon laser irradiation of the anterior surface of the iris.

Methods: Iris and aqueous hyaluronan concentrations in rabbit were measured with a radiometric assay at different time points after laser irradiation.

Results: Total hyaluronan content in iris tissue increased 3-fold to a peak concentration of 71-72 microg/g at 1 and 2 days after laser treatment.

Synthesis of hyaluronan by normal and wounded rabbit iris.

Background: Endogenous hyaluronan has been found in different tissues in the normal and traumatized eye. However, the main source, the biological aspects and the full potential role of hyloronan are still unclear.

Methods: Hyaluronan production was studied both in organ culture and in vivo, using a double-label protocol with [35S]sulfate and [3H]glucosamine.

Endogenous hyaluronan in the normal and traumatized rabbit iris.

Purpose: Hyaluronan concentration in the iris tissue after iridectomy was studied in order to establish the influence of trauma on the production of hyaluronan in the anterior chamber of the eye and whether the iris participates in this process or not.

Method: Hyaluronan was measured with a radiometric assay at different time points after surgery and identified with specific histochemical staining.

Results And Conclusions: The concentration of hyaluronan in iris tissue peaked two days after surgery, reaching an average of 115.