Effect of Andrographis paniculata extract on progesterone in blood plasma of pregnant rats.

The effect of the powdered extract of Andrographis paniculata leaves (APE), an active principle of Kan Jang tablets [standardized for content of andrographolide (4.6%) and 14-deoxo-andrographolide (2.3%) content (total andrographolids--6.

[N-acetylglutamate-5-phosphotransferase of the thermophilic bacterium Bacillus stearothermophilus: nucleotide sequence of the gene and enzyme characterization].

A nucleotide sequence of the argB gene of strain Bacillus stearothermophilus NCIB 8224 was determined. The argB gene codes for N-acetylglutamate-5-phosphotransferase of 258 amino acids with a molecular weight of 26918 D. This value is in good agreement with the SDS-PAG electrophoresis gata for identification of the heat stable B.

[Molecular cloning and structural-functional analysis of the arginine biosynthesis genes of the thermophilic bacterium Bacillus stearothermophilus].

Genes encoding arginine biosynthesis of Bacillus stearothermophilus strain 718 were cloned in the mutant argA strain of the Escherichia coli K-12. The arg genes were shown to be located on the 3.7 kb DNA fragment in the following order: argA--argE--argB.

[Restriction mapping of recombinant plasmids carrying the genes for arginine biosynthesis in Escherichia coli K-12].

ArgA and argECBH genes of Escherichia coli K-12 were cloned on the pBR322 vector. Restriction maps of the recombinant plasmids were constructed. Deletion mutants of these recombinant plasmids, retaining the functional argA and argE genes, were obtained using different restriction enzymes.