Hormonal induction and seasonal variation in male reproductive viability of the Southern Rocky Mountain boreal toad.

The Southern Rocky Mountain boreal toad (Anaxyrus boreas boreas) depends on both the rearing of wild-collected egg masses and a long-standing conservation breeding program (CBP), the latter of which heavily relies on assisted reproductive technologies (ARTs) to support wild populations. Achieving consistent reproductive success in the CBP, however, remains a significant challenge. Natural breeding has not led to a sustained increase in reproductive capacity, prompting the exploration of exogenous hormone treatments as an alternative strategy.

Epigenetic silencing and blockade of latency reversal by an HIV-1 encoded antisense transcript.

The mechanisms that regulate human immunodeficiency virus 1 (HIV-1) latency are not fully elucidated. We reported that an HIV-1 antisense transcript (AST) induces epigenetic modifications at the HIV-1 promoter causing a closed chromatin state that suppresses viral transcription. Here we show that ectopic expression of AST in CD4+ T-cells from people with HIV-1 under antiretroviral therapy blocks latency reversal in response to pharmacologic and T-cell receptor stimulation, enforcing transcriptional silencing.

Impact of UV pre-treatment on the Longissimus thoracis et lumborum muscle proteomes of dry-aged beef cuts: A characterisation within two sampling locations.

This research aimed to explore the changes in two sampling locations (internal and external) of the Longissimus thoracis et lumborum (LTL) beef muscle proteomes subjected to ultraviolet light before dry-aging. It further compared the biological processes and associated proteins at interplay at the external locations of UV pre-treated and control dry-aged samples. Before dry-aging, proteins related to external stimuli were differentially abundant between both locations possibly due to the early post-mortem energy metabolism attempting to compensate for energy deficiencies and stress derived from slaughter and processing.

Author Correction: Single-cell transcriptomics stratifies organoid models of metabolic dysfunction-associated steatotic liver disease.

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Research priorities: Establishing discipline-based education research in prosthetics and orthotics.

Background: Education research, as a field of study, is relatively new to the health professions. Research and scholarship in prosthetics and orthotics education is limited, and the priority areas and needs of individuals interested in pursuing this area of research and scholarship are unknown.

Objectives: The objectives of this study were to collaboratively identify the areas in which education research in prosthetics and orthotics is needed, to prioritize these areas, and to evaluate the resources needed to support those interested in pursuing education research.