In vitro dissolution of encapsulated or dispersed Vitamin e and cholesterol is correlated with preservation of refrigerated ovine sperm.

Background: Vitamin E ( -tocopherol) and cholesterol are crucial components in cellular protection and physiological processes. Their uses in biological media face challenges due to their poor solubility and stability.

Objective: The study investigated the complex interactions of these bioactive compounds in various encapsulation systems of cyclodextrin and liposome, as well as dispersion in PEG-6000, in an attempt to improve the viability, motility, and preservation of ovine sperm cells.

Drug release, stability and efficiency of vitamin e loaded in liposomes for bovine sperm protection in cryopreservation medium.

Background: It is known that a considerable number of drugs in clinical use or under development are water-insoluble drugs with poor bioavailability. The liposomal delivery system has drawn attention as one of the noteworthy approaches to increase both dissolution and absorption because of its biocompatibility and ability to encapsulate hydrophobic molecules in the lipid domain. However, several drawbacks have been reported, the most common is liposome structural instability.

Rosmarinus officinalis Essential Oil Preloaded in β-Cyclodextrin: Effect on Ram Spermatozoa Motility, Membrane Integrity and Oxidative Status During 4°C Storage.

Background: Rosmarinus officinalis essential oil (Rom) has been reported recently to be of interest for use in sperm cryopreservation. However, related to its lipophilic characteristics, encapsulation in cyclodextrin could enhance Rom positive effects by increasing its solubility in sperm extenders.

Objective: To compare the effect of Rom preloaded in hydroxypropyl-β-cyclodextrin (Rom-cd) to Rom alone (Rom) on ram sperm conserved at 4°C.

The Complementary Effect of Cholesterol and Vitamin E Preloaded in Cyclodextrins on Frozen Bovine Semen: Motility Parameters, Membrane Integrity and Lipid Peroxidation.

Background: During cryopreservation sperm cells suffer from two major deleterious impacts: oxidative stress and cold shock.

Objective: To investigate in bovine species the benefit of cholesterol and vitamin E, both loaded in cyclodextrins, as a double protection against oxidative stress and cold shock.

Materials And Methods: Semen was collected from nine mature bulls using an artificial vagina and each ejaculate was split into four equal aliquots.