Age-dependent changes of presynaptic neuromodulation via A1-adenosine receptors in rat hippocampal slices.

The presynaptic neuromodulation of stimulation-evoked release of [3H]-acetylcholine by endogenous adenosine, via A1-adenosine receptors, was studied in superfused hippocampal slices taken from 4-, 12- and 24-month-old rats. 8-Cyclopentyl-1,3-dimethylxanthine (0.25 microM), a selective A1-receptor antagonist, increased significantly the electrical field stimulation-induced release of [3H]-acetylcholine in slices prepared from 4- and 12-month-old rats, showing a tonic inhibitory action of endogenous adenosine via stimulation of presynaptic A1-adenosine receptors.

Effect of diet on tissue protease activity.

Rats 1, 3, 12, and 24 months old were fed diets low in protein (8% casein), and proteolytic activity in tissue from brain, liver, and lung was determined. After a low-protein diet was fed for 4 weeks to 1-month-old rats, there was a significant increase in cathepsin D activity in liver, and calpain activity was increased in lung. Little change was seen in proteolytic activity in brain.

Differential changes in presynaptic modulation of transmitter release during aging.

The purpose of this study was to assess the functional role of presynaptic alpha 2-autoreceptors in noradrenergic transmission in the hippocampus and dopamine-2 heteroreceptors in cholinergic transmission in the striatum in young, adult, and senescent rats. Male and female Wistar rats (4, 12, and 24 months old) were used and the release of radioactivity from striatal and hippocampal slices that had been loaded either with [3H]choline or with [3H]norepinephrine was measured at rest and in response to field stimulation (2 Hz, 360 shocks). The release was challenged by sulpiride, a selective dopamine-2 receptor antagonist, and CH-38083, a selective alpha 2-adrenoceptor antagonist.

Histologic and cytogenetic effects of redentin on the spermiogenesis and bone marrow cells of the mouse, an in vivo experiment.

The chromosomes in bone marrow cells and spermatocytes of Redentin (1 X 20 mg/kg, po)-treated CFLP strain male mice were examined. Spermiogenesis was checked histologically. On the basis of the results, it can be stated that Redentin, at this experimental dosage, did not induce chromosome aberrations nor did it damage spermiogenesis.