Preparation and properties of chloroplasts depleted of chloroplast coupling factor 1 by sodium bromide treatment.

Chloroplasts were treated with 2 m sodium bromide. The resulting particles lost their ATPase activity and chloroplast coupling factor 1 subunits were detected in the supernatant by means of gel electrophoresis and specific antibodies. The chloroplast coupling factor 1 depleted particles show high rates of Hill reaction with pH optimum shifted toward lower pH.