Experimental studies on the protective efficacy of a Pseudomonas aeruginosa vaccine.

Pseudomonas aeruginosa vaccine (PV) containing cell proteins with molecular weight (Mr) 20,000-100,000 and up to 0.08% (w/v) admixture of lipopolysaccharide was obtained by water-salt extraction and subsequent ultrafiltration. PV protects mice against experimental P.

[The growth and protein A accumulation of Staphylococcus aureus A-676 on a medium made from nonfood raw material].

In this research the experimental analytical method of balancing culture media, based on the determination of the yield of the target product, i.e. staphylococcal protein A, has been used.

[Increase in the coefficient of component utilization by balancing the composition of the nutrient medium for growing pneumococci].

The possibility of using the experimental-analytical balance method (EABM) for development of balanced media, optimal and economic by their composition is shown. The method is based on the specific growth activity of the medium components and mathematical calculation of their concentrations. A balanced medium containing human placenta hydrolysate was developed.

[Dissolved oxygen content in microbiological nutrient media].

The modification of a highly sensitive method for the determination of the concentration of dissolved oxygen in liquid culture media and in water is described. This modification permits the easy conversion of the relative readings of electrode membrane transducers for measuring the partial pressure of oxygen into the readings characterizing the absolute concentration of oxygen in the medium (mg/cu. dm).

[Nutrient medium for the isolation and cultivation of pneumococcus].

A culture medium for the isolation and cultivation of pneumococci, produced in a solid or liquid form and based on raw material unsuitable for use as foodstuff (human placenta), has been developed. The amino acid composition of the medium has been studied. The medium has been found to contain 19 amino acids, to be free from ballast serum proteins and blood, and to ensure the good growth of pneumococci isolated from pathological material, the formation of the normal capsule, as well as active biological properties.