Comparison of the inhibitory effect of E-prostaglandins in human and rabbit platelet-rich plasma and washed platelets.

1. The anti-aggregatory potency of a number of E-type PGs was compared in human and rabbit platelet-rich plasma (PRP) and washed platelets. The potency of 13,14-dihydro-PGE1 and 5,6-dihydro-PGE3 is significantly higher in human than in rabbit washed platelets, while the potency of 15-keto-13,14-dihydro-PGE1 is higher in rabbit.

Is prostacyclin a major prostaglandin produced by endothelial cells in culture?

Prostaglandin biosynthetic activity of cultured human aortic and umbilical vein endothelial cells (passages 3-9) and of human aortic subendothelial cells (passage 0) was studied. Labelled arachidonic acid or endoperoxide H2 (final concentrations, 10 microM and 3.5 microM, respectively) were used as prostaglandin synthesis precursors.