Hematopoietic Stem Cell Transplantation During the Era of COVID-19 in Queen Rania Children's Hospital.

Background: Corona virus disease 2019 (COVID-19) is causing a health crisis nowadays, and all countries are following the recommendations of the WHO to decrease the spread of the disease. Till now, few data are available regarding the clinical course, severity of the disease and the duration of infectivity of COVID-19 in patients received Hematopoietic Stem Cell Transplantation (HSCT).

Objective: To evaluate the medical protocols and outcome of patients who underwent HSCT during the pandemic of COVID-19.

Methods Used for Detection and Recovery of Escherichia coli O157:H7 Associated with a Food-Borne Disease Outbreak .

The current Food Safety and Inspection Service method for detection and recovery of Escherichia coli O157:H7, (including modified EC broth with novobiocin (mEC+n) and a direct blot ELISA). was used to analyze beef and environmental samples during an investigation of a food-borne disease outbreak attributed to consumption of undercooked hamburger patties. Double-modified trypticase soy broth (dmTSB) and a commercially available dipstick immunoassay were also used to improve detection/recovery of E.

Presence of Yersinia enterocolitica Serotype O:5,27 in Slaughter Pigs.

The tonsils, tongue, mesenteric lymph nodes, cecal contents, and feces from 50 slaughter pigs were evaluated for the presence of pathogenic Yersinia enterocolitica . The cecal contents and feces of two pigs were positive (4%) with the pathogenic serotype O:5,27, biotype 3, whereas all other pigs were negative for pathogenic serotypes of Y. enterocolitica .

Effect of Freezing on Infectivity of Toxoplasma Gondii Tissue Cysts in Pork.

To study the effect of low temperatures on infectivity of Toxoplasma gondii tissue cysts, pork from infected pigs was mixed with infected mouse brains and homogenized thoroughly. Twenty-gram samples of infected homogenized meat which were sealed in plastic pouches, pressed to a uniform thickness resulting in samples having the dimensions of ~.2 × 16 × 18 cm, were subjected to temperatures of -1 to -171.

Infectivity of Trichinella spiralis from Frozen Pork.

This research was carried out to determine the time/temperature exposure of Trichinella spiralis to freezing conditions necessary to destroy the infectivity of the trichinae. Experimentally infected pork was subjected to temperatures of -1 to -193°C for one sec to 182 d and the treated pork samples, which contained about 1000 larvae per gram of tissue, were subjected to rat bioassay to determine infectivity of the larvae. A linear regression equation, logt = 5.