Structural and Mechanistic Characterization of the Flavin-Dependent Monooxygenase and Oxidase Involved in Sorbicillinoid Biosynthesis.

Sorbicillinoids are yellow secondary metabolites synthesized through an elegant combination of enzymatic and spontaneous biochemical processes. The flavin-dependent monooxygenase SorC and oxidase SorD are crucial in this interplay, enabling the generation of a diverse array of functionally complex sorbicillinoids. By solving the crystal structures of SorC and SorD from with sorbicillin bound in the active site, we describe the catalytically active binding conformations, crucial for attaining enantioselective and stereoselective control in these enzymatic reactions.

Operando Photoemission Imaging of the Energy Landscape from a 2D Material-Based Field-Effect Transistor.

As the integration of transition metal dichalcogenides (TMDC) becomes more advanced for optoelectronics, it is increasingly relevant to develop tools that can correlate the structural properties of the materials with their electrical output. To do so, the determination of the electronic structure must go beyond the hypothesis that the properties of the pristine material remain unaffected after the device integration, which generates changes in the dielectric environment, including electric fields that are likely to renormalize the electronic spectrum. Here, we demonstrate that nanobeam photoemission spectroscopy is a well-suited tool to unveil the device energy landscape under operando conditions.

Comprehensive phenotypic analysis of multiple gene deletions of α-glucan synthase and Crh-transglycosylase gene families in highlighting the versatility of the fungal cell wall.

Multiple paralogs are found in the fungal genomes for several genes that encode proteins involved in cell wall biosynthesis. The genome of contains five genes encoding putative α-1,3-glucan synthases (AgsA-E) and seven genes encoding putative glucan-chitin crosslinking enzymes (CrhA-G). Here, we systematically studied the effects of the deletion of single ( or ), double ( and , or all five genes () present in .

Evaluation of Cytotoxic and Antioxidant Potential of Green-Synthesized Silver and Gold Nanoparticles From Nepeta leucophylla Benth.

In the present study, silver (AgNPs) and gold (AuNPs) nanoparticles (NPs) have been prepared using the ethanolic extract of Nepeta leucophylla (NLe). This plant was considered owing to its richness in natural polyphenols and antioxidants that are well known for their reducing potential. Different techniques, such as UV (ultraviolet-visible spectrophotometer), FTIR (Fourier transform infrared radiation), and XRD (X-ray diffraction), were utilized for the characterization of NPs.