Impact of Photoactivated Curcumin on Proliferation of Experimentally Induced Periodontitis and Diabetic Gingival Fibroblast Cell Line.

Objective: To evaluate the impact of photoactivated curcumin on proliferation of experimentally induced periodontitis and diabetic gingival fibroblast in laboratory settings.

Materials And Methods: Gingival fibroblast (GF) cells were divided into healthy GF (HGF), diabetic GF (DGF), and periodontitis-associated diabetic GF (P-DGF) cells that were treated with a solution of curcumin that was prepared and diluted in autoclave distilled water to obtain a concentration used (1 mg/ml). Gingival fibroblasts with curcumin and without curcumin were seeded in a 96-well plate that was treated with a light-emitting diode curing light with a wavelength of 445 nm using a transparent diffuser tip.

Molecular grammars of intrinsically disordered regions that span the human proteome.

Intrinsically disordered regions (IDRs) of proteins are defined by functionally relevant molecular grammars. This refers to IDR-specific non-random amino acid compositions and non-random patterning of distinct pairs of amino acid types. Here, we introduce GIN (Grammars Inferred using NARDINI+) as a resource, which we have used to extract the molecular grammars of all human IDRs and classified them into thirty distinct clusters.

Nuclear speckle proteins form intrinsic and -dependent microphases.

Nuclear speckles are enriched in serine / arginine rich splicing factors (SRSFs), such as SRSF1. Splicing factors and proteins such as TDP-43 concentrate into distinct speckle territories to enable pre-mRNA processing. We have discovered that SRSFs and TDP-43 are block copolymers and the protein-specific interplay of inter-block repulsions and attractions drives spontaneous microphase separation.

Myopericytoma of the liver: A rare tumor - A case report.

Introduction: Myopericytoma is an uncommon benign neoplasm composed of myoid-appearing perivascular cells. It had been reported in various soft tissues, but only few case reports were found reported in liver.

Case Presentation: We reported a case of myopericytoma in a 43-year-old female with a history of a left adnexal cyst and foul-smelling per vaginal discharge with no significant findings in per abdominal examination.

Cardiopulmonary exercise testing as an integrative approach to explore physiological limitations in Duchenne muscular dystrophy.

Background: Cardiopulmonary exercise testing (CPET) is the gold-standard for quantification of peak oxygen uptake (VO) and cardiorespiratory and muscle responses to exercise. Its application to Duchenne muscular dystrophy (DMD) has been scarce due to the notion that muscle weakness inherent to disease restricts the cardiorespiratory system from reaching maximal capacity.

Objective: To investigate the utility of CPET in DMD by 1) establishing whether patients can perform maximal-effort exercise for valid VO peak assessment; 2) quantifying VO peak repeatability; 3) characterizing muscle and cardiorespiratory responses; 4) comparing VO peak to 6-min walk distance (6MWD).