CRISPR-CLEAR: Nucleotide-Resolution Mapping of Regulatory Elements via Allelic Readout of Tiled Base Editing.

CRISPR tiling screens have advanced the identification and characterization of regulatory sequences but are limited by low resolution arising from the indirect readout of editing via guide RNA sequencing. This study introduces , an end-to-end experimental assay and computational pipeline, which leverages targeted sequencing of CRISPR-introduced alleles at the endogenous target locus following dense base-editing mutagenesis. This approach enables the dissection of regulatory elements at nucleotide resolution, facilitating a direct assessment of genotype-phenotype effects.

Promoting dual careers at higher education institutions: 31 benefits ranked by the project Student Athletes Erasmus+ Mobility in Europe (SAMEurope).

Introduction: The project Student Athletes Erasmus+ Mobility in Europe identified and defined a total of 31 benefits offered to dual-career student athletes who are combining their university studies with high-level training. The project was co-funded by the European Union and carried out by five universities: Chalmers University of Technology (Sweden), Institut national des sciences appliquées de Lyon (France), University of Jyväskylä (Finland), Karlsruhe Institute of Technology (Germany), and Universitat Jaume I (Spain).

Methods: The purpose of the study was to rank these benefits by combining the perspectives of the university staff and the student athletes from each university in the consortium.

JAK-STAT signaling maintains homeostasis in T cells and macrophages.

Immune cells need to sustain a state of constant alertness over a lifetime. Yet, little is known about the regulatory processes that control the fluent and fragile balance that is called homeostasis. Here we demonstrate that JAK-STAT signaling, beyond its role in immune responses, is a major regulator of immune cell homeostasis.

Sulfate sensitivity of aquatic organism in soft freshwaters explored by toxicity tests and species sensitivity distribution.

Elevated concentrations of sulfate in waterways are observed due to various anthropogenic activities. Elevated levels of sulfate can have harmful effects on aquatic life in freshwaters: sulfate can cause osmotic stress or specific ion toxicity in aquatic organisms, especially in soft waters where Ca and Mg concentrations are low. Formerly, chronic toxicity test data in soft water have been scarce.

Hydrogeological Characterization of Crystalline Bedrock Using Borehole Magnetic Resonance.

We aimed to test borehole magnetic resonance (BMR) method for determining hydraulic parameters (porosity, permeability, and hydraulic conductivity) required for hydrogeological modeling in two distinct crystalline rock environments. These sites comprise Proterozoic basement rocks of different compositions: mafic rocks at the Sakatti mining development site in northern Finland and felsic rocks at the Olkiluoto Island nuclear repository site in southwest Finland. Although BMR is widely used for determining storage and hydraulic properties in sedimentary environments, there have been few studies in crystalline bedrocks.