A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies.

A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and actinomycin D. Using this approach we produced tetradomas secreting bispecific MAbs to horseradish peroxidase and human alpha-fetoprotein.

Monoclonal antibodies to different epitopes of human alpha-fetoprotein (AFP).

Monoclonal antibodies specific for human alpha-fetoprotein (AFP) were produced by the hybridoma technique. By using solid-phase immunofluorescence and radioimmunoassays, the antibodies were proven to distinguish 4 different epitopes on the AFP molecule.