[The influence of the penetrating cryoprotectors on protein synthesis in the cell-free system of the rat liver].

The influence of the penetrating cryoprotector and Mg2+ ions on the protein-synthesizing activity of postmitochondrial supernantant of the rat liver as well as on aminoacylation processes has been investigated. The addition of the penetrating cryoprotectors--ethylene glycol and DMSO--resulted in the concentration-dependant reversible inhibition of the protein biosynthesis and aminoacylation reaction in the cell-free system. These cryoprotectors at low concentrations intensified the stimulating effect of Mg2+ on the cumulative protein synthesis in the cell-free system.

[Stimulation of cell cultures recovery after cryopreservation by the cattle cord blood FRACTION (below 5 kDa) or Actovegin].

The capacities of the cattle cord blood low-molecular fraction (below 5 kDa) and Actovegin (the vealer blood fraction (below 5 kDa)) for recovering functions of cell cultures after cryopreservation compared. Their influence proliferation of the flozen-thawed cell cultures, certain stages of their growth, cell attachment, rate of cell spreading, and mitotic regiment has been studied. Both the cord blood low-molecular fraction and Actovegin were shown to stimulate growth of the cell cultures after cryopreservation more efficiently at the concentration of 224 μg/ml.

[Stimulatory effect of the cord blood fraction below 5 kDa and "Actovegin" on the growth of continuous cell lines].

The influence of the cattle cord blood fraction with molecular weight of components below 5 kDa on adhesive and proliferative properties of continuous cell lines BHK-21 clone q3/04 and PK-15-IECVM was investigated in comparison with the preparation "Actovegin". It was shown that adding this fraction as well as "Actovegin" to growth media did not affect the efficiency of culture attachment, promoted culture spreading, improved cell morphology and stimulated mitotic activity of the cultures. The efficiency of the cord blood fraction below 5 kDa estimated by the induces studied was found to be higher than the "Actovegin" efficiency when cells being cultivated in the media different contents of blood serum.

[Changes in protein composition in the goldfish tissues depending on season and temperature of keeping].

Data of SDS-electrophoresis of tissues proteins of the goldfish Carassius auratus netted in winter and summer and kept at different temperatures (+5 degrees C and +20 degrees C) are presented. It has been ascertained that after keeping the "winter" fish at 5 degrees C for 2 weeks quantities of the protein 205 kD in the red muscles and heart and the protein 172 kD in the liver are significantly decreased. A slight decline in contents of the proteins 105 and 45 kD was observed in the white muscles of deacclimated fish.

[Glycosaminoglycanes of cartilage tissue of rats after the mechanical trauma: the influence of low-molecular fraction from the cord blood and blood of dairy calves].

Influence of low-molecular fraction (to 5 kDa) from cord blood (FCB) compared with "Actovegin" derived from the blood of veal calves on the content of the matrix polysaccharide components from and proteins of the cartilage connecting tissue after the mechanical trauma was investigated. It is established, that intramuscular administration of FCB essentially stimulates the accumulation of the basic components of the matrix: hexosamine, uronic acid, hyaluronic acid, chondroitinsulfate and heparin, and also the most important components of structural proteins in the cartilage regenerate of the rat knee joint.