Production of Fully Capped mRNA for Transfection into Mammalian Cells: A Protocol for Enzymatic Degradation of Uncapped Transcripts After In Vitro mRNA Synthesis.

The functionality of messenger RNA, such as stability and translation, is determined by several elements. In Eukaryotes, the 5' end of the mRNA is modified to contain a 5' cap structure, the presence of which protects the mRNA from degradation by 5' to 3' exoribonucleases and promotes mRNA translation. The in vitro synthesis of RNA has recently attracted ample attention for its application as a source of therapeutic agents or research tools.

The strain-dependent cytostatic activity of Lactococcus lactis on CRC cell lines is mediated through the release of arginine deiminase.

Background: Colorectal cancer (CRC) is one of the most commonly diagnosed cancers, posing a serious public health challenge that necessitates the development of new therapeutics, therapies, and prevention methods. Among the various therapeutic approaches, interventions involving lactic acid bacteria (LAB) as probiotics and postbiotics have emerged as promising candidates for treating and preventing CRC. While human-isolated LAB strains are considered highly favorable, those sourced from environmental reservoirs such as dairy and fermented foods are also being recognized as potential sources for future therapeutics.

Inhibition of RNA Polymerase III Augments the Anti-Cancer Properties of TNFα.

Tumour necrosis factor alpha (TNFα) is a multifunctional cytokine that plays a pivotal role in apoptosis, cell survival, as well as in inflammation and immunity. Although named for its antitumor properties, TNFα also has tumour-promoting properties. TNFα is often present in large quantities in tumours, and cancer cells frequently acquire resistance to this cytokine.