Publications by authors named "A Juhasz Szalai"
The intricate interplay between DNA and proteins is key for biological functions such as DNA replication, transcription and repair. Dynamic nanoscale observations of DNA structural features are necessary for understanding these interactions. Here we introduce graphene energy transfer with vertical nucleic acids (GETvNA), a method to investigate DNA-protein interactions that exploits the vertical orientation adopted by double-stranded DNA on graphene.
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- The study investigates the energy transfer properties of 2D titanium carbide MXene materials and explores their use in single-molecule biosensing for the first time.
- DNA origami structures are employed to precisely position single dye molecules on the MXene surface, allowing for controlled experiments on energy transfer efficiency.
- Findings demonstrate that MXenes can serve as effective nanorulers for measuring distances at the molecular level, showcasing their potential for biosensing applications that require high sensitivity to small distance changes.
Article Synopsis
- RhoA has a dual role in axon development, acting both as an inhibitor and a promoter, depending on the timing and specific downstream signaling pathways involved.
- In undifferentiated neurons, RhoA activity is concentrated in growth cones, while in developing axons, it shows a biphasic pattern: low in nascent axons and heightened in elongating ones.
- Different RhoA signaling pathways influence axon growth: RhoA-ROCK signaling prevents axon initiation but doesn’t affect elongation, whereas RhoA-mDia signaling enhances elongation by stabilizing microtubules in growth cones.
Purpose: The aim of this study was to assess whether complement proteins C3 and C4 are produced by immortalized human conjunctival epithelial (HCjE) cells.
Methods: Supernatants and cell lysates from undifferentiated and differentiated HCjE cells were assayed for C3 and C4 by enzyme-linked immunosorbent assay. To measure complement protein function, supernatants and lysates were treated with heat-aggregated IgG, and soluble C5b-9 was measured.
The world of 2D materials is steadily growing, with numerous researchers attempting to discover, elucidate, and exploit their properties. Approaches relying on the detection of single fluorescent molecules offer a set of advantages, for instance, high sensitivity and specificity, that allow the drawing of conclusions with unprecedented precision. Herein, it is argued how the study of 2D materials benefits from fluorescence-based single-molecule modalities, and vice versa.
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