Publications by authors named "A Jonckheere"
Primary human mast cells (MC) obtained through culturing of blood-derived MC progenitors are the preferred model for the study of MRGPRX2- IgE-mediated MC activation. In order to assess the impact of culture conditions on functional MRGPRX2 expression, we cultured CD34-enriched PBMC from peripheral whole blood (PB) and buffy coat (BC) samples in MethoCult medium containing stem cell factor (SCF) and interleukin (IL)-3, modified through variations in seeding density and adding or withholding IL-6, IL-9 and fetal bovine serum (FBS). Functional expression of MRGPRX2 was assessed after 4 weeks via flow cytometry.
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- Intense physical exercise in adolescents can lead to exercise-induced bronchoconstriction (EIB), which affects 22% of athletes studied, with many being unaware of their condition.
- The study involved 327 adolescent athletes who underwent various tests, revealing links between EIB, asthma history, and atopy, with many EIB athletes not using inhalation therapy.
- Effective screening methods like questionnaires and lung function tests were highlighted, emphasizing the need for better detection of EIB, particularly in athletes exposed to air pollution.
Article Synopsis
- Obesity is linked to various health issues, including non-alcoholic fatty liver disease and asthma, but there is limited research on how exercise and diet affect these conditions in the context of obesity.
- In an experiment with mice, those on a high-fat diet were split into two groups: one continued on the high-fat diet while the other switched to a normal diet, with half of each group doing exercise.
- The results showed that while combining diet and exercise reduced liver fat and damage, it increased airway inflammation and sensitivity in mice, highlighting a complex relationship between weight management and respiratory health.
Background: The hit-hard-and-early (HHAE) strategy where treatment is initiated with high-efficacy therapies opposed to low-efficacy therapies presents a potential paradigm shift in multiple sclerosis (MS) management. This study aimed to assess the adoption of the HHAE strategy in Germany and the United States (US) from 2020 to 2022 based on real-world data.
Methods: The analysis was based on longitudinal, patient-level data from Germany and the US.
The authors report the natural history of three patients with late-diagnosed Classic Galactosemia (CG) (at 16, 19 and 28 years). This was due to a combination of factors: absence of neonatal screening, absence of some typical acute neonatal symptoms, and negative galactosemia screening. This report underlines the value of neonatal screening and the importance of further diagnostic testing in case of late-onset manifestations.
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