Proteomic Profiling of Extracellular Vesicles in Inflammatory Bowel Diseases.

The proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology of Crohn's disease (CD) and ulcerative colitis (UC), as well as for biomarker discovery. To characterize the proteomic composition of serum EVs in patients with CD and UC to identify biomarkers and molecular pathways associated with pathogenesis and activity. Methods: Serum EVs were enriched and analyzed in patients with quiescent CD, active CD (aCD), quiescent UC, active UC (aUC), and healthy controls (HCs) (n = 30 per group).

Virus-Mimicking Polymer Nanocomplexes Co-Assembling HCV E1E2 and Core Proteins with TLR 7/8 Agonist-Synthesis, Characterization, and In Vivo Activity.

Hepatitis C virus (HCV) is a major public health concern, and the development of an effective HCV vaccine plays an important role in the effort to prevent new infections. Supramolecular co-assembly and co-presentation of the HCV envelope E1E2 heterodimer complex and core protein presents an attractive vaccine design strategy for achieving effective humoral and cellular immunity. With this objective, the two antigens were non-covalently assembled with an immunostimulant (TLR 7/8 agonist) into virus-mimicking polymer nanocomplexes (VMPNs) using a biodegradable synthetic polyphosphazene delivery vehicle.

Clinical and immunological features of specific antibody deficiency in a pediatric hospital in Colombia.

Introduction. Specific antibody deficiency is an innate error of humoral immunity characterized by normal levels of immunoglobulin isotypes, recurrent infections, and a reduced reaction to polysaccharide antigens in vaccines. Objective.

Shallow groove technique in sextant pattern femtosecond laser-assisted cataract surgery.

Femtosecond laser-assisted cataract surgery (FLACS) is a useful technology for anterior segment surgery that creates cleavage planes via photodisruption of ocular tissue. Compared to conventional cataract surgery, FLACS has been thought to potentially improve surgical precision, accuracy, and reproducibility. Its introduction into cataract surgery has enabled the development of new techniques and has been purported to be potentially beneficial in improving surgical safety and providing quicker visual rehabilitation.