Publications by authors named "A J Racher"
Article Synopsis
- Researchers enhanced the biosynthetic ability of CHO cells by evolving Lonza's proprietary CHOK1SV® cells through over 150 generations at a lower temperature of 32°C.
- The cold-adapted cells showed significant improvements in growth, biomass, and mitochondrial function, along with higher productivity of certain antibodies and proteins while maintaining proper protein processing.
- This transformation was linked to substantial genomic changes, indicating that CHO cells can adapt significantly to environmental conditions, making directed evolution a promising strategy for improving protein production capabilities.
Article Synopsis
- Chinese hamster ovary (CHO) cells are crucial for producing monoclonal antibodies and other complex biotherapeutics using metabolic selection marker technologies like glutamine synthetase (GS) and dihydrofolate reductase (DHFR).
- A new selection marker system based on CHO cells' need for proline was developed using pyrroline-5-carboxylase synthetase (P5CS), which allowed engineered cells to thrive in proline-free conditions, comparable to standard CHO cell growth in proline-rich environments.
- By combining the P5CS and GS selection systems, researchers successfully created CHO cell lines that improved recombinant protein expression, leading to higher yields of a challenging monoclonal antibody during production.
In recent years, the stability of biotherapeutics in vivo has received increasing attention. Assessing the stability of biotherapeutics in serum may support the selection of adequate molecule candidates. In our study, we compared the physical stability of 8 different monoclonal antibodies (mAbs) in phosphate-buffered saline (PBS) and human serum.
View Article and Find Full Text PDFThe data presented in this article relates to the manuscript entitled 'Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production', published in the Journal Metabolic Engineering [1]. In the article here, we present data examining the overexpression of the lipid metabolism modifying genes and in CHO cells by densitometry of western blots and by using mass spectrometry to investigate the impact on specific lipid species. We also present immunofluorescence data at the protein level upon SCD1 and SREBF1 overexpression.
View Article and Find Full Text PDFChinese hamster ovary (CHO) cell expression systems have been exquisitely developed for the production of recombinant biotherapeutics (e.g. standard monoclonal antibodies, mAbs) and are able to generate efficacious, multi-domain proteins with human-like post translational modifications at high concentration with appropriate product quality attributes.
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