Expanding the flooding in Landsat across tidal systems model to Landsat 5-9 imagery for long-term marsh inundation analysis.

Tidal flooding can significantly impact vegetation pixel reflectance of coastal salt marshes, presenting a problem for remote sensing studies of these highly productive ecosystems. The current study aimed to spatially and temporally expand our previously developed Flooding in Landsat Across Tidal Systems (FLATS) model to detect and analyze the long-term changes in flooded marsh pixels in Landsat 5-9 imagery. As the FLATS index is only calibrated for Landsat 8, our goal was to expand the use of FLATS to a greater range of Landsat imagery and facilitate the masking of flooded pixels in long-term time series of vegetation indices.

Regionalized cell and gene signatures govern esophageal epithelial homeostasis.

Regionalized disease prevalence is a common feature of the gastrointestinal tract. Herein, we employed regionally resolved Smart-seq3 single-cell sequencing, generating a comprehensive cell atlas of the adult mouse esophagus. Characterizing the esophageal axis, we identify non-uniform distribution of epithelial basal cells, fibroblasts, and immune cells.

Multimodal analyses of immune cells during bone repair identify macrophages as a therapeutic target in musculoskeletal trauma.

Musculoskeletal traumatic injuries (MTI) involve soft tissue lesions adjacent to a bone fracture leading to fibrous nonunion. The impact of MTI on the inflammatory response to fracture and on the immunomodulation of skeletal stem/progenitor cells (SSPCs) remains unknown. Here, we used single-nucleus transcriptomic analyses to describe the immune cell dynamics after bone fracture and identified distinct macrophage subsets with successive pro-inflammatory, pro-repair and anti-inflammatory profiles.

Distinct origin and region-dependent contribution of stromal fibroblasts to fibrosis following traumatic injury in mice.

Fibrotic scar tissue formation occurs in humans and mice. The fibrotic scar impairs tissue regeneration and functional recovery. However, the origin of scar-forming fibroblasts is unclear.