Dual-Stage Cross-Flow Filtration: Integrated Capture and Purification of Virus-Like Particles.

Virus-like particles (VLPs) are a versatile technology for the targeted delivery of genetic material through packaging and potential surface modifications for directed delivery or immunological issues. Although VLP production is relatively simple as they can be recombinantly produced using microorganisms such as Escherichia coli, their current downstream processing often relies on individually developed purification strategies. Integrating size-selective separation techniques may allow standardized platform processing across VLP purification.

Unveiling adipose populations linked to metabolic health in obesity.

Precision medicine is still not considered as a standard of care in obesity treatment, despite a large heterogeneity in the metabolic phenotype of individuals with obesity. One of the strongest factors influencing the variability in metabolic disease risk is adipose tissue (AT) dysfunction; however, there is little understanding of the link between distinct cell populations, cell-type-specific transcriptional programs, and disease severity. Here, we generated a comprehensive cellular map of subcutaneous and visceral AT of individuals with metabolically healthy and unhealthy obesity.

TRPM7 activity drives human CD4 T-cell activation and differentiation in a magnesium dependent manner.

T lymphocyte activation is a crucial process in the regulation of innate and adaptive immune responses. The ion channel-kinase TRPM7 has previously been implicated in cellular Mg homeostasis, proliferation, and immune cell modulation. Here, we show that pharmacological and genetic silencing of TRPM7 leads to diminished human CD4 T-cell activation and proliferation following TCR mediated stimulation.

A Computational Approach for Internal Tendon Routing Channels in a Tendon-Driven Continuum Joint.

Tendon-driven continuum soft robots are currently applied in research and are given a promising perspective for future applications. For the routing of the tendons from the actuator to the point where the loading is demanded, two routing possibilities exist in the literature: internal routing of the tendons with the help of structurally embedded Bowden sheaths and external tendon routing where the tendon is not in contact with the soft structure. The application of the latter is a clear disadvantage for applications due to the high risk of interference with the tendon, for example, causing the tendon to break.

Technological barriers to providing pediatric mental and behavioral healthcare in emergency departments.

In the United States, about 5% of pediatric Emergency Department (ED) visits involve mental and behavioral health (MBH) emergencies, and EDs are often ill-equipped to provide timely, appropriate care, leading to prolonged stays and increased risks of trauma and harm for these children and their families. This study investigated technological barriers affecting safe and effective pediatric mental and behavioral healthcare in emergency departments through observational studies and semi-structured interviews with 55 medical professionals across four ED settings: pediatric MBH unit, pediatric ED, and adult ED. A total of 12 barrier themes were identified through the thematic analysis of the interviews relating to technology use that impacts the care of pediatric MBH patients.