2D Dose Reconstruction by Artificial Neural Network for Pretreatment Verification of IMRT Fields.

The use of intensity-modulated radiation therapy (IMRT) is developing rapidly in clinical routines. Because of the high complexity and uniqueness of IMRT treatment plans, patient-specific pretreatment quality assurance is generally considered a necessary prerequisite for patient treatment. In this work, we proposed a modified methodology of electronic portal imaging device (EPID)-based dose validation for pretreatment verification of IMRT fields by applying artificial neural networks (ANNs).

SiRNA-mediated IGF-1R inhibition sensitizes human colon cancer SW480 cells to radiation.

Purpose: Insulin like growth factor receptor 1 (IGF-1R) is well-documented to play a key role in radiation response and tumor radiosensitivity, thus offering an attractive clinic drug target to enhance tumor sensitivity to anti-cancer radiotherapy.

Material And Methods: Human colon carcinoma SW480 cells were transfected with the specific small interference RNA (siRNA) expression vector (pkD-shRNA-IGF-1R-V2) designed to target IGF-1R mRNA. The expression of IGF-1R mRNA and its protein among the transfected and untransfected cells were detected by semi-quantitative RT-PCR and ELISA assay.

Efficiency of adult mouse spermatogonial stem cell colony formation under several culture conditions.

The aim of this study was to compare the in vitro effects of glial cell line-derived neurotrophic factor, stem cell factor, granulocyte macrophage-colony stimulating factor, and co-culture with Sertoli cells on the efficiency of adult mouse spermatogonial stem cells colony formation. For these purpose, both Sertoli and spermatogonial cells were isolated from adult mouse testes. The identity of the cells was confirmed through analysis of alkaline phosphatase activity, immunocytochemistry against OCT-4, c-kit, and vimentin, and also by transplantation of these cells in the recipient testes.

The morphological changes of adult mouse testes after 60Co gamma-Radiation.

Background: Cytotoxic therapy can lead to prolonged azoospermia or even sterility. In the present study, we investigated the morphological changes of mouse testes after gamma-Radiation.

Methods: After anaesthetizing of NMRI mice, testes and their surrounding tissues were irradiated using a cobalt therapy machine.