Publications by authors named "A J Ambegaonkar"
Article Synopsis
- Memory B cells (MBCs) are essential for defending against similar and variant pathogens by becoming either plasma cells or germinal center B cells.
- Human MBCs can be categorized into switched IgG and unswitched IgM, but their responses to B cell receptor activation are not fully understood.
- Research indicates that IgG MBCs only respond to high-affinity stimuli and favor plasma cell development, while IgM MBCs can be eliminated by high-affinity stimuli but can differentiate into germinal center B cells with low-affinity triggers, highlighting their distinct yet complementary roles.
Article Synopsis
- Mapping 3D plasma membrane topology in live cells provides important insights into cell biology, utilizing advanced imaging techniques for better visualization.
- Traditional methods like 3D-SIM improve resolution but struggle with nanoscale details; new axial interferometry techniques can pinpoint protein locations near the membrane.
- The newly developed MAxSIM, combined with a height-controlled mirror system, enhances imaging accuracy and allows for detailed, real-time 3D mapping of plasma membranes in live cells.
The world's struggle to contain the SARS-CoV-2 epidemic, primarily through vaccination, has highlighted the importance of better understanding the biology of B cells that participate in defense against infectious diseases, both acute and chronic. Here, we focus on a population of human B cells, termed atypical B cells (ABCs), that comprise a distinct B-cell lineage that differentiates from naive B cells in an interferon-γ-driven process, and are infrequent in healthy individuals but significantly expanded in chronic infectious diseases, including malaria, as well as in systemic autoimmune diseases such as systemic lupus erythematosus (SLE). Recent comparisons of ABCs by single-cell RNAseq provided evidence that ABCs in diverse chronic infectious diseases and in systemic autoimmune diseases are highly related and share common drivers of differentiation and expansion.
View Article and Find Full Text PDFBackground: Communication difficulties are one of the primary symptoms associated with dementia, and mobile applications have shown promise as tools for facilitating communication in patients with dementia (PwD). The literature regarding mobile health (mHealth) applications, especially communications-based mHealth applications, is limited.
Objective: This review aims to compile the existing literature on communications-based mobile applications regarding dementia and assess their opportunities and limitations.
Chronic infectious diseases have a substantial impact on the human B cell compartment including a notable expansion of B cells here termed atypical B cells (ABCs). Using unbiased single-cell RNA sequencing (scRNA-seq), we uncovered and characterized heterogeneities in naïve B cell, classical memory B cells, and ABC subsets. We showed remarkably similar transcriptional profiles for ABC clusters in malaria, HIV, and autoimmune diseases and demonstrated that interferon-γ drove the expansion of ABCs in malaria.
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