Intraspecific divergence of diploid grass is associated with structural chromosome changes.

Smith in Sibthorp et Smith, 1806 is diploid grass with MM genome constitution occurring mainly in Greece. Two morphologically distinct subspecies - Chennaveeraiah, 1960 and (Holzmann ex Boissier) Eig, 1929 are discriminated within , however, genetic and karyotypic bases of their divergence are not fully understood. We used Fluorescence in situ hybridization (FISH) with repetitive DNA probes and electrophoretic analysis of gliadins to characterize the genome and karyotype of to assess the level of their genetic diversity and uncover mechanisms leading to radiation of subspecies.

Chromosome and Molecular Analyses Reveal Significant Karyotype Diversity and Provide New Evidence on the Origin of .

Zhuk. is tetraploid grass species (2n = 4x = 28, UUXX) closely related to and growing in Western Asia and a western part of the Fertile Crescent. Genetic diversity of was assessed using C-banding, FISH, nuclear and chloroplast (cp) DNA analyses, and gliadin electrophoresis.

The feasibility of employing a home healthcare model for education and treatment of opioid overdose using a naloxone auto-injector in a private practice pain medicine clinic.

Objectives: The purpose of this study was to determine if employing a home healthcare model for education and treatment of opioid overdose using the Evzio (Naloxone) auto-injector in a private practice pain clinic.

Methods: A prospective survey was used to determine the feasibility of integrating a naloxone auto-injector within the patient's home with a home care training model. Twenty moderate or high-risk patients were enrolled from the chronic pain clinic.

Genetic classification of Aegilops columnaris Zhuk. (2n=4x=28, UUXX) chromosomes based on FISH analysis and substitution patterns in common wheat × Ae. columnaris introgressive lines.

Aegilops columnaris is a tetraploid species originated from Ae. umbellulata (2n=2x=14, UU) and a yet unknown diploid grass species. Although Ae.

Determinants of Patient Satisfaction in a Private Practice Pain Management Clinic.

Background: Patient satisfaction is used to measure physician performance in hospital and governmental practice settings. There is limited understanding about factors affecting satisfaction in a chronic pain management setting for patients prescribed chronic opioids.

Objective: To identify the determinants of patient satisfaction and correlation with recommended outcome measures in a private practice pain management clinic.