[A DEVICE FOR THE VACUUM TREATMENT OF HISTOLOGICAL MATERIAL].

A device to carry out all types of vacuum infiltration in a wide temperature range. The device is easy to use and allows you to simultaneously conduct the infiltration of a large number of tissue samples. The main element of the device is a temperature-controlled cuvette, the temperature of which is set in the range of 23-65 °C.

The Allium test: injection of test compounds into the bulb.

We developed three methods for injecting substances into the bulb of Allium: (1) direct injection, (2) injection through the open space in the bulb after the removal of the upper half of the bulb, and (3) through a special "tunnel" cut in the bulb. Water (control) and solutions of methotrexate and copper sulfate were injected into bulbs and the dynamics of root growth were measured. We found that 2.

Analysis of the metabolites in apical area of Allium cepa roots by high resolution NMR spectroscopy method.

Elucidation of the molecular mechanisms determining the formation of various tissues and organs is one of the central problems of cell biology. High-resolution NMR spectroscopy was applied for the analysis of the metabolites produced at the various areas of the apical part of the onion Allium cepa roots. To this end, three samples were extracted from the root apex (the root cap, the meristem region and the cell elongation zone).

[Micromorphometric analysis of root cap cells in Allium cepa].

A two-dimensional micromorphometric analysis of a root cap in Allium cepa included the measurement of the areas of about six thousand cell profiles on both longitudinal and transversal sections. Basing on the results of this analysis, quantitative descriptions of two cell populations from the columella and periferic part of the root cap have been created. The scheme of arrangement of root cap cells, located at a distance of 250 microm or less from a root tip, has been developed.