[Placental alkaline phosphatase as a marker of malignant neoplasms].

Activity of placental alkaline phosphatase (PAP) was studied in blood serum of 53 healthy persons and of 72 oncologic patients, using solid-phase immunoenzymatic analysis with polyclonal antibodies towards PAP. The enzyme was detected both in blood serum of healthy persons and of oncologic patients. The blood serum under study was preheated at 65 degrees in order to inactivate the intestinal phosphatase--the only isoenzyme cross-reacting with antibodies to thermostable PAP.

[Effectiveness of methods of isolating specific class-G rabbit antibodies for the immunoenzyme determination of human placental alkaline phosphatase].

Four different chromatographic methods of IgG isolation from rabbit antisera to placental alkaline phosphatase (HPAP) have been compared. The antibodies were obtained by ion-exchange chromatography and affinity chromatography on protein-A-sepharose, on the sepharose with immobilized antigen. IgG samples were characterized by the content of specific antibodies to HPAP and checked in enzyme immunoassay (EIA).