Publications by authors named "A I Ruberto"
The production of energy in the form of ATP by the mitochondrial ATP synthase must be tightly controlled. One well-conserved form of regulation is mediated via ATPase inhibitory factor 1 (IF1), which governs ATP synthase activity and gene expression patterns through a cytoprotective process known as mitohormesis. In apicomplexans, the processes regulating ATP synthase activity are not fully elucidated.
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- - Malaria, caused by the Plasmodium parasite, faces challenges due to drug resistance, prompting the need for new treatments targeting both symptomatic and asymptomatic liver stages; a cost-effective luciferase detection method for liver stage screening has been developed using common lab reagents.
- - The study optimized a protocol for testing liver stage parasites, validating it with 28 existing anti-malarials to ensure reliable signal data, enabling the screening of the Global Health Priority Box (GHPB) for potential drug candidates.
- - Results showed that the optimized screening method produced a more stable luciferase signal with lower cell density, identifying 9 promising hits with selective activity against the P. berghei liver stages.
The production of energy in the form of ATP by the mitochondrial ATP synthase must be tightly controlled. One well-conserved form of regulation is mediated via ATPase inhibitory factor 1 (IF1), which governs ATP synthase activity and gene expression patterns through a cytoprotective process known as mitohormesis. In apicomplexans, the processes regulating ATP synthase activity are not fully elucidated.
View Article and Find Full Text PDFVesiviruses are important animal pathogens with a broad host range, and they have also been involved in accidental contamination of cells used for the production of drugs for rare and life-threatening human diseases. A vesivirus (family Caliciviridae) was detected in minks (Neovison vison) with respiratory and neurological signs, during syndromic surveillance for SARS-CoV-2 conducted in Italy. The complete genome (8,397 nucleotides in length) of the vesivirus strain ITA/2021/mink/TE (OR130287) was obtained by combining NGS approach with 5' and 3' RACE protocols.
View Article and Find Full Text PDFSingle-cell genomics provide researchers with tools to assess host-pathogen interactions at a resolution previously inaccessible. Transcriptome analysis, epigenome analysis, and immune profiling techniques allow for a better comprehension of the heterogeneity underlying both the host response and infectious agents. Here, we highlight technological advancements and data analysis workflows that increase our understanding of host-pathogen interactions at the single-cell level.
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