Correlation between carbon flux through the pentose phosphate pathway and production of the antibiotic methylenomycin in A3(2).

Radiorespirometry was employed to study carbon metabolism during the growth of A3(2) in a minimal medium which permitted the production of methylenomycin as the sole detectable secondary metabolite. A switch in the pattern of carbon metabolism from the Embden-Myerhof-Parnas pathway to the pentose phosphate pathway occurred during the period of slower growth in batch culture which immediately preceded entry into the stationary phase. This coincided with the period of methylenomycin production.

An integrated approach to studying regulation of production of the antibiotic methylenomycin by Streptomyces coelicolor A3(2).

A physiological and molecular biological study was made of the control of methylenomycin biosynthesis by Streptomyces coelicolor A3(2). A simple and reliable assay for this antibiotic was developed. Conditions that permit the synthesis of methylenomycin by S.