Identification by methods of steady-state and kinetic spectrofluorimetry of endogenous porphyrins and flavins sensitizing the formation of reactive oxygen species in cancer cells.

The question about acceptor molecules of optical radiation that determine the effects of photobiomodulation in relation to various types of cells still remains the focus of attention of researchers. This issue is most relevant for cancer cells, since, depending on the parameters of optical radiation, light can either stimulate their growth or inhibit them and lead to death. This study shows that endogenous porphyrins, which have sensitizing properties, may play an important role in the implementation of the effects of photobiomodulation, along with flavins.

Design and Realization of Polymeric Waveguide/Microring Structures for Telecommunication Domain.

Polymer-based micro-optical components are very important for applications in optical communication. In this study, we theoretically investigated the coupling of polymeric waveguide and microring structures and experimentally demonstrated an efficient fabrication method to realize these structures on demand. First, the structures were designed and simulated using the FDTD method.

Effect of continuous wave, quasi-continuous wave and pulsed laser radiation on functional characteristics of fish spermatozoa.

For the first time, using sturgeon sperm as a model system, sensitive to optical radiation, the comparative studies of biological effect of continuous wave, quasi-continuous wave, nano- and picosecond laser radiation under conditions with equal average irradiance (3 mW/cm) and wavelength (532 nm) have been carried out. Analyzing the parameters of spermatozoa motion it has been shown that, depending on the energy dose and mode of laser operation, the radiation may have both stimulatory and inhibitory effect on the velocity of motion and spermatozoa motility duration as well as on sustaining of functional characteristics of cold-stored sperm. The possibility of increasing the fertilization rate due to use of the sperm preliminary treated with laser radiation is demonstrated.

Comparative Effect of Low-intensity Laser Radiation in Green and Red Spectral Regions on Functional Characteristics of Sturgeon Sperm.

A comparative study of the effect of low-intensity laser radiation in green (λ = 532 nm) and red (λ = 632.8 nm) spectral regions at equal average irradiance (3 mW cm ) on functional characteristics of Siberian sturgeon spermatozoa is carried out. Confirmation of the photobiomodulation effect of the radiation is obtained by analyzing spermatozoa motility, percentage of motile spermatozoa and fertilization rate.