Allogeneic stem cell transplantation for severe acquired aplastic anaemia using a fludarabine-based preparative regimen.

We reviewed our experience in the treatment of 13 patients with severe acquired aplastic anaemia, using a newly developed non-myeloablative regimen consisting of fludarabine (total dose 180 mg/m2), cyclophosphamide (total dose 120 mg/kg), and antithymocyte globulin (total dose 40 mg/kg). All except one patient received multiple transfusions and had failed prior immunosuppressive treatment. Twelve out of 13 patients achieved sustained engraftment.

[The descending cortical pathways of the frontal lobe to the nuclei of the hypothalamic mamillary bodies in human craniocerebral trauma].

Structural organization of conducting ways of the frontal lobe cortex to hypothalamic nuclei was studied in sections of brain from 5 patients who died in short terms after craniocerebral trauma and during the experiment in 5 macaque rhesus monkeys with unilateral destruction of different cortical fields of frontal area. Series of brain sections were processed using silver nitrate impregnation after Bielschowsky [correction of Bilshovsky] with counterstaining of nuclear structures after Kavamura with cresyl violet. The presence of direct corticohypothalamic ways from the cortex of the orbital surface of inferior (field 47 and its subfields) and superior (field 11) frontal gyri and frontal pole (field 10) to nuclei of mamillary complex and lateral hypothalamus was established.

Recovery of infectious human immunodeficiency virus type 1 after fusion of defectively infected clones of U-937 cells.

Polyethylene glycol was used to induce polykaryon formation among U-937 cell subclones carrying defective human immunodeficiency virus (HIV) type 1 proviral DNA. Fusion of cells which produced gp120-defective virions (UHC15.7) with cells unable to generate reverse transcriptase (RT) activity (UHC8 and UHC18) yielded polykaryons which made infectious viral progeny that showed normal protein profiles.

High frequency of isolation of defective human immunodeficiency virus type 1 and heterogeneity of viral gene expression in clones of infected U-937 cells.

Limiting-dilution techniques were employed to derive single-cell clones from U-937 cells that had been chronically infected with human immunodeficiency virus type 1. All clones thus obtained were positive for the presence of viral antigens; however, not all of the clones produced infectious progeny virus, as detected by the presence of reverse transcriptase (RT) activity in culture fluids. Six of these clones were monitored over time to determine whether their phenotype of human immunodeficiency virus type 1 expression was stable.