Combination of cyclophosphamide and double-stranded DNA demonstrates synergistic toxicity against established xenografts.

Background: Extracellular double-stranded DNA participates in various processes in an organism. Here we report the suppressive effects of fragmented human double-stranded DNA along or in combination with cyclophosphamide on solid and ascites grafts of mouse Krebs-2 tumor cells and DNA preparation on human breast adenocarcinoma cell line MCF-7.

Methods: Apoptosis and necrosis were assayed by electrophoretic analysis (DNA nucleosomal fragmentation) and by measurements of LDH levels in ascitic fluid, respectively.

Identification of cancer stem cells and a strategy for their elimination.

It has been established previously that up to 40% of mouse CD34(+) hematopoietic stem cells are capable of internalizing exogenous dsDNA fragments both in vivo and ex vivo. Importantly, when mice are treated with a combination of cyclophosphamide and dsDNA, the repair of interstrand crosslinks in hematopoietic progenitors is attenuated, and their pluripotency is altered. Here we show for the first time that among various actively proliferating mammalian cell populations there are subpopulations capable of internalizing dsDNA fragments.

Vitrification with DAP 213 and cryotop of ex situ and in situ feline cumulus-oocyte complexes.

This study was undertaken to compare cryotolerance, in terms of viability and resumption of meiosis after warming and culture (24 and 48 h), of ex situ (isolated) and in situ (enclosed in the ovarian tissue) feline cumulus-oocyte complexes (COCs) vitrified with DAP 213 (2 m DMSO, 1 m acetamide, 3 m propylene glycol) in cryotubes or Cryotop method. Ovaries were harvested from 49 pubertal queens. Of each pair of ovaries, one was dissected to release COCs randomly divided into three groups: fresh COCs (control), ex situ COCs vitrified with DAP 213 and Cryotop.

Quantitative morphology of experimental fibrosis in canine pancreas after laser tunneling.

Dogs with experimental fibrosing pancreatitis were subjected to laser tunneling to assess its effect on the dynamics of some morphometric indices of the pancreas. On post-tunneling day 60, volume ratio of the parenchyma significantly increased while the volume ratio of the stroma decreased in comparison to the dogs with fibrosing pancreatitis not corrected by laser tunneling.

Changed morphine sensitivity of morphine-dependent rats after laser exposure of the cerebral prefrontal cortex.

We studied the effect of bilateral laser exposure of the cerebral prefrontal cortex in morphine-dependent rats on their sensitivity to positive reinforcing and toxic effect of morphine. Laser exposure led to partial deafferentation of the cingulum. Exposure of the anterior and central areas of the prefrontal cortex stimulated self-administration of low morphine doses and had no effect on self-administration of high concentrations.