Characteristics of transplanted mouse myeloproliferative disease developed after repeated injections of granulocytic colony-stimulating factor.

Transplanted myeloproliferative disease developed in mice against the background of repeated injections of granulocytic CSF was characterized using morphological and molecular biological methods. It was demonstrated that transplanted myeloproliferative disease had a non-viral nature and is probably induced by repeated injections of granulocytic CSF. Tumor cells actively populate the liver of sick animals, which leads to their rapid death.

Genetic analysis reveals different roles of Schizosaccharomyces pombe sfr1/dds20 in meiotic and mitotic DNA recombination and repair.

DNA double-strand break (DSB) repair mediated by the Rad51 pathway of homologous recombination is conserved in eukaryotes. In yeast, Rad51 paralogs, Saccharomyces cerevisiae Rad55-Rad57 and Schizosaccharomyces pombe Rhp55-Rhp57, are mediators of Rad51 nucleoprotein formation. The recently discovered S.

Five RecA-like proteins of Schizosaccharomyces pombe are involved in meiotic recombination.

The genome of Schizosaccharomyces pombe contains five genes that code for proteins with sequence similarity to the Escherichia coli recombination protein RecA: rad51+, rhp55+, rhp57+, rlp1+, and dmc1+. We analyzed the effect of deletion of each of these genes on meiotic recombination and viability of spores. Meiotic recombination levels were different from wild type in all recA-related mutants in several genetic intervals, suggesting that all five RecA homologs of S.