Publications by authors named "A Huckriede"
Introduction: Complex respiratory models, including air-liquid interface (ALI) transwell cultures and airway organoids, have emerged as promising tools for studying human respiratory virus infections. These models address several limitations of conventional two-dimensional cell line and animal models. However, the lack of standardized protocols for the application of these models in infection studies limits the possibilities for comparing results across different research groups.
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- In vitro systems using human peripheral blood mononuclear cells (PBMCs) can effectively evaluate vaccine responses, particularly focusing on B cells alongside T cells and antigen-presenting cells.
- The study investigated how B cells in PBMCs respond to different influenza vaccine formulations (whole inactivated virus vs. split virus) and the impact of the adjuvant CpG ODN 2395 on immune responses.
- Findings revealed that WIV stimulated better B cell differentiation and higher antibody production compared to SIV, with the combination of WIV and CpG leading to the most significant immune responses, highlighting the potential of using PBMCs in early vaccine evaluations.
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- Increasing resistance to current antiviral drugs for influenza highlights the need for new treatments, focusing on both drug development and administration methods.
- CD-6'SLN, a novel entry inhibitor that mimics sialic acid, was formulated into a dry powder using spray drying with a dispersion enhancer, ensuring stability and efficacy in a dry powder inhaler.
- In tests on infected mice, the aerosolized CD-6'SLN significantly reduced disease severity and inflammation compared to control groups, demonstrating its potential as a new treatment for influenza.
RSV infection remains a serious threat to the children all over the world, especially, in the low-middle income countries. Vaccine delivery via the mucosa holds great potential for inducing local immune responses in the respiratory tract. Previously, we reported the development of highly immunogenic RSV virus-like-particles (RSV-VLPs) based on the conformationally stable prefusogenic-F protein (preFg), glycoprotein and matrix protein.
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- Antibody responses from COVID-19 vaccinations are decreasing, especially with the rise of the Omicron variant, prompting the need for new bivalent mRNA booster vaccines containing both ancestral and Omicron spike proteins.
- A study evaluated how different initial vaccination regimens (priming) influenced the effectiveness of these bivalent boosters, revealing that while the boosters increased neutralizing antibodies and T-cell responses, they were less effective against the newer XBB.1.5 variant.
- The findings support using vaccines tailored to current circulating strains for vulnerable populations and stress the need for ongoing monitoring of immune responses to inform future vaccination strategies.