[Pertussis in a 4-month-old infant with unrecognized cystic fibrosis].

We present a case report of a small boy with cystic fibrosis complicated by pertussis and pneumothorax. The child had not been vaccinated against Bordetella pertussis due to the failure to thrive and was infected with the bacterium at the age of 4 months. The course of the disease was severe, with respiratory distress and spontaneous pneumothorax.

[Disseminated infection with Mycobacterium avium in a patient with newly diagnosed HIV infection].

In the Czech Republic, mycobacteriosis is relatively rare. The low incidence probably reflects high BCG coverage rates in the Czech population. Globally, the importance of BCG vaccine has been increasing, as a result of acquired immunodeficiencies, particularly HIV infection.

"Mycobacterium avium subsp. hominissuis" in neck lymph nodes of children and their environment examined by culture and triplex quantitative real-time PCR.

"Mycobacterium avium subsp. hominissuis" often causes cervical lymphadenitis in children; its prompt and accurate identification enables adequate therapy, tracing, and prevention. The aims of this study were to determine the causative agent of lymphadenitis using culture, PCR, and triplex quantitative real-time PCR (qPCR) methods with DNA directly isolated from tissue, as well as to identify possible sources of infection from the environment.

[Abscessing lymphadenitis in a 1.5-year-old boy].

At present, Bartonella species are increasingly important as infectious agents in both animals and humans. Bartonella henselae, the most frequently diagnosed species, is known to cause numerous clinical syndromes in both immunocompetent and immunocompromised patients. In healthy individuals, the infection is most commonly manifested as the so-called cat scratch disease.

[Can the determination of IgG antibodies to the pertussis toxin help the diagnosis of the disease?].

Background: In the differential diagnosis of protracted irritating cough we should always consider the possibility of pertussis. Serology performed primarily in a late stage of the disorder does not always provide a clear answer. We wanted to verify whether a quantitative determination of IgG antibodies to the pertussis toxin (IgG-PT) could help establish a clear diagnosis.