Understanding barriers to diabetes eye screening in a large rural general practice: an audit of patients not reached by screening services.

Introduction Diabetes is a leading cause of blindness in New Zealanders, yet a significant proportion of patients with diabetes are not reached by diabetes eye screening. Aim Our study audited patients with diabetes in a large New Zealand rural general practice, to identify addressable barriers preventing patients from attending diabetes eye screening. Methods All patients who had missed a diabetes eye screening appointment at the Dargaville Hospital Eye Screening Clinic between 2018 and 2021 were identified.

An evaluation of a novel device for measuring eating, rumination, and inactive behaviors in lactating Holstein dairy cattle.

Validation of precision dairy-monitoring technologies establishes technology behavioral-monitoring efficacy for research and commercial application. Technology metrics should be associated with behaviors of known physiological importance. The objective of this research project was to evaluate the Nedap SmartTag Neck (Nedap Livestock Management, Groenlo, the Netherlands) for dairy cow behavior measuring accuracy.

2-D Western blotting for evaluation of antibodies developed for detection of host cell protein.

Recombinant proteins generated for therapeutic use must be substantially free of residual host cell protein (HCP). The presence of host cell protein (HCP) is usually assayed by ELISA using a polyclonal antibody mixture raised against a population of proteins derived from the host cell background. This antibody should recognize as high a proportion as possible of the potential HCPs in a given sample.

Fractionation of complex protein mixtures by liquid-phase isoelectric focusing.

Protein fractionation is essential to uncovering low-abundance proteins in complex protein mixtures. Many common methods and techniques are used to fractionate proteins, including chromatography (size exclusion, affinity, ion exchange, etc.), electrophoresis, and solution chemistry.

Random herd curves in a test-day model for milk, fat, and protein production of dairy cattle in The Netherlands.

With random regression models, genetic parameters of test-day milk production records of dairy cattle can be estimated directly from the data. However, several researchers that used this method have reported unrealistically high variances at the borders of the lactation trajectory and low genetic correlations between beginning and end of lactation. Recently, it has been proposed to include herd-specific regression curves in the random regression model.