Publications by authors named "A Hafiza"
Article Synopsis
- Tuberculosis is the oldest human pathogen and continues to be a major cause of death from bacterial infections, with 7.5 million new cases reported in 2022, the highest since the WHO began monitoring in 1995.
- The spike in cases in 2022 is likely linked to disruptions caused by the COVID-19 pandemic, raising public health concerns.
- A transcriptome analysis of different strains identified 6,898 differentially expressed genes related to virulence, transport, and cell wall biosynthesis, providing valuable data for future drug development against tuberculosis.
Prenatal diagnosis is essential in the new era of diagnosis and management of genetic diseases in obstetrics. Multiple ligation-dependent probe amplification (MLPA) is a recent technique for prenatal diagnosis for the relative quantification of 40 different nucleic acid sequences in one single reaction. We had utilized the MLPA technique in detecting aneuploidies in amniotic fluid samples from 25 pregnant women from the Obstetrics and Gynaecology Department UKMMC, versus the quantitative fluorescent polymerase chain reaction (QF-PCR) method.
View Article and Find Full Text PDFAlpha (Α) thalassaemia is the most common inherited disorder in Malaysia. The clinical severity is dependant on the number of Α genes involved. Full blood count (FBC) and haemoglobin (Hb) analysis using either gel electrophoresis, high performance liquid chromatography (HPLC) or capillary zone electrophoresis (CE) are unable to detect definitively alpha thalassaemia carriers.
View Article and Find Full Text PDFParaproteinemia is one of the diagnostic features of multiple myeloma. A commonly used method is the detection of paraprotein by agarose gel electrophoresis (AGE) followed by by immunofixation electrophoresis (IFE) to confirm monoclonality. Due to their smaller size, immunoglobulin A (IgA) and light chain only paraproteins may appear at the beta or even alpha 2 protein fractions.
View Article and Find Full Text PDFObjective: The capillary electrophoresis (CE) is a new system that utilizes the principle of electrokinetic separation of molecules in eight electrolyte buffer-filled silica capillaries. In this study, we established the normal ranges of haemoglobin A2 (HbA2) and haemoglobin F (HbF) levels for normal individuals using this system and also the HbA2 level in beta thalassaemia and haemoglobin E (HbE) individuals.
Materials And Methods: 154 samples from normal individuals, 218 samples from beta thalassaemia heterozygotes and 91 samples from HbE heterozygotes were subjected to high performance liquid chromatography (HPLC) and CE analysis.