Publications by authors named "A H Velders"
Detection of spp. DNA in gynaecological samples by quantitative real-time polymerase chain reaction (qPCR) is considered to be the reference diagnostic test for female genital schistosomiasis (FGS). However, qPCR needs expensive laboratory procedures and highly trained technicians.
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- Current whole sporozoite (SPZ) vaccines show limited effectiveness in malaria-endemic regions, possibly due to reduced immune responses from prior malaria exposure.
- Researchers have developed a method to enhance SPZ vaccine immunogenicity by attaching a TLR7 agonist-based adjuvant, leading to significantly higher immune responses, including a 35-fold increase in pro-inflammatory IL-6 production.
- Mice immunized with this enhanced SPZ vaccine demonstrated improved production of specific immune cells, suggesting that this chemical augmentation strategy could make malaria vaccines more effective.
Nuclear Magnetic Resonance (NMR) spectroscopy is a most powerful molecular characterization and quantification technique, yet two major persistent factors limit its more wide-spread applications: poor sensitivity, and intricate complex and expensive hardware required for sophisticated experiments. Here we show NMR with a single planar-spiral microcoil in an untuned circuit with hyperpolarization option and capability to execute complex experiments addressing simultaneously up to three different nuclides. A microfluidic NMR-chip in which the 25 nL detection volume can be efficiently illuminated with laser-diode light enhances the sensitivity by orders of magnitude via photochemically induced dynamic nuclear polarization (photo-CIDNP), allowing rapid detection of samples in the lower picomole range (normalized limit of detection at 600 MHz, nLOD, of 0.
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- A comprehensive analysis of the elemental and molecular makeup of PO (plant species) and AN revealed many metabolites like carbohydrates and amino acids through NMR spectroscopy.
- The study used advanced NMR techniques, analyzing both live plant parts and residues, to identify bioactive compounds, particularly in PO residues.
- NMR spectroscopy showcased its effectiveness in plant metabolomics by identifying biomarkers and offering molecular fingerprints for better understanding and potential recycling uses, like creating fertilizer.
Background: Surgically induced nerve damage is a common but debilitating side effect. By developing tracers that specifically target the most abundant protein in peripheral myelin, namely myelin protein zero (P0), we intend to support fluorescence-guided nerve-sparing surgery. To that end, we aimed to develop a dimeric tracer that shows a superior affinity for P0.
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